An illustration of the polymerase chain reaction (PCR). Step 1: Solution is heated to 95°C
to denature ("unzip") the two strands of the target DNA (A). Step 2: Solution
is cooled to ~55°C to allow the primers to anneal (bind) to the ends of the
DNA strands (B). Step 3: Solution is reheated to ~75°C to allow
TAQ polymerase to synthesize complementary copies of each strand.
Related Links